Advanced glycation end-products accumulation compromises embryonic development and achievement of pregnancy by assisted reproductive technology

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Masao Jinno1,*, Masayoshi Takeuchi2, Aiko Watanabe1, Koji Teruya3, Jun Hirohama1, Noriko Eguchi1 and Aiko Miyazaki4

1Women's Clinic Jinno, 3-1-39-201 Kokuryou-chou, Choufu City, Tokyo 182-0022, Japan

2Department of Life Pharmacy, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa City, Ishikawa, Japan

3Department of Public Health, School of Health Sciences, Kyorin University, Hachioji City, Tokyo, Japan

4HUSHIMI Pharmatheuticals, Kagawa, Japan *Correspondence address. Tel: +81-424-80-3105; Fax: +81-424-80-3105; E-mail: mjinno{at}s9.dion.ne.jpReceived August 20, 2010. Revision received December 2, 2010. Accepted December 10, 2010. BACKGROUND Advanced glycation end-products (AGE) are pivotal in aging and diabetes. Aging and polycystic ovary syndrome, a diabetes-associated disease, often cause infertility. We examined how AGE accumulation affects assisted reproductive technology (ART) outcomes.

METHODS In this retrospective analysis, toxic AGE (TAGE), pentosidine (Pent) and carboxymethyl lysine (CML) in blood and follicular fluid (FF) were measured in 157 ART-patients. We analyzed associations of AGE with ART outcomes and pre-ART clinical factors.

RESULTS TAGE, Pent and CML in FF and TAGE in serum, showed significant negative correlations with estradiol and numbers of follicles larger than 12 mm in diameter, retrieved oocytes, fertilized oocytes and embryos. AGE, Pent in FF and TAGE in serum showed significant negative correlations with ongoing pregnancy. Areas under receiver-operating characteristic curves for AGE (0.709), Pent in FF (0.686) and TAGE in serum (0.667) were significantly larger than for the reference (0.5). Women with serum TAGE above 7.24 U/ml showed decreased oocyte numbers and ongoing pregnancy rates, even with younger age or lower Day-3 FSH. Serum TAGE correlated positively with leptin (R = 0.51), BMI, low-density lipoprotein, triglyceride, glucose, homeostasis model assessment-insulin resistance and insulin.

CONCLUSIONS Serum TAGE and FF Pent accumulations correlated highly with poor follicular and embryonic development and with a lower likelihood of ongoing pregnancy. Serum TAGE predicts poor ART outcomes independent of age and Day-3 FSH.

© The Author 2011. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oup.com This ArticleHum. Reprod. (2011) 26 (3): 604-610. doi: 10.1093/humrep/deq388 First published online: January 12, 2011

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Constitutional change of Italian legislation on assisted reproduction technology improves pregnancy rate especially in older patients

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Paola Scaruffi1, Maria Rita Primavera1, Luiza Helena Abbamonte1, Shanti Levi1, Caterina De Leo2, Annamaria Nicoletti1 and Paola Anserini1,*

1Center of Physiopathology of Human Reproduction, Department of Obstetrics and Gynecology, ‘San Martino’ Hospital, L.go R. Benzi, 10, 16132 Genova, Italy

2Department of Obstetrics and Gynecology, University of Genova, Genova, Italy *Correspondence address. Tel: +39-010-5555843; Fax: +39-010-5556909; E-mail: paola.anserini{at}hsanmartino.itSir,

In February 2004, the Italian Parliament approved a law (namely 40/2004) regulating assisted reproduction technology ( Benagiano and Gianaroli, 2004). This law imposed many limitations on Italian reproductive specialists. The most important of these restrictions was the provision that no more than three oocytes could be fertilized at one time during an IVF treatment, since all embryos obtained had to be transferred simultaneously. On May 2009, the Italian Constitutional Court outlawed some restrictions set out in the 40/2004 law ( Benagiano and Gianaroli, 2010). The most important point of the ruling is that embryo protection is limited by the imperative to ensure a concrete possibility to achieve a successful pregnancy. The Court also strongly reaffirmed the supremacy of the physician's judgment in carrying out a full evaluation and a personalized treatment plan for each woman and couple. Therefore, Italian reproductive specialists can now define an individualized optimal number of embryos to give the best chance of achieving a pregnancy while limiting the number of cryopreserved embryos.

The recently published article by Levi Setti et al. (2010) reported a retrospective analysis of 3274 IVF cycles, comparing fresh cycles before and after the 40/2004 law was modified. No significant difference was found between the two groups in terms of patients’ age, basal FSH levels, …

This ArticleHum. Reprod. (2011) 26 (4): 959-961. doi: 10.1093/humrep/der014 First published online: February 11, 2011

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Constitutional change of Italian legislation on assisted reproduction technology improves pregnancy rate especially in older patients

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Paola Scaruffi1, Maria Rita Primavera1, Luiza Helena Abbamonte1, Shanti Levi1, Caterina De Leo2, Annamaria Nicoletti1 and Paola Anserini1,*

1Center of Physiopathology of Human Reproduction, Department of Obstetrics and Gynecology, ‘San Martino’ Hospital, L.go R. Benzi, 10, 16132 Genova, Italy

2Department of Obstetrics and Gynecology, University of Genova, Genova, Italy *Correspondence address. Tel: +39-010-5555843; Fax: +39-010-5556909; E-mail: paola.anserini{at}hsanmartino.itSir,

In February 2004, the Italian Parliament approved a law (namely 40/2004) regulating assisted reproduction technology ( Benagiano and Gianaroli, 2004). This law imposed many limitations on Italian reproductive specialists. The most important of these restrictions was the provision that no more than three oocytes could be fertilized at one time during an IVF treatment, since all embryos obtained had to be transferred simultaneously. On May 2009, the Italian Constitutional Court outlawed some restrictions set out in the 40/2004 law ( Benagiano and Gianaroli, 2010). The most important point of the ruling is that embryo protection is limited by the imperative to ensure a concrete possibility to achieve a successful pregnancy. The Court also strongly reaffirmed the supremacy of the physician's judgment in carrying out a full evaluation and a personalized treatment plan for each woman and couple. Therefore, Italian reproductive specialists can now define an individualized optimal number of embryos to give the best chance of achieving a pregnancy while limiting the number of cryopreserved embryos.

The recently published article by Levi Setti et al. (2010) reported a retrospective analysis of 3274 IVF cycles, comparing fresh cycles before and after the 40/2004 law was modified. No significant difference was found between the two groups in terms of patients’ age, basal FSH levels, …

This ArticleHum. Reprod. (2011) 26 (4): 959-961. doi: 10.1093/humrep/der014 First published online: February 11, 2011

View the original article here

Peliculas Online

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